FIGURE

FIGURE 5

ID

ZDB-FIG-201223-5

Publication

Han et al., 2020 - Inhibition of Embryonic HSP 90 Function Promotes Variation of Cold Tolerance in Zebrafish

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FIGURE 5

Roles of atg9b and psmd12 in cold tolerance in zebrafish cells. (A,D) zebrafish ZF4 cells were treated with 5 μM radicicol for indicated times, then the mRNA levels of atg9b and psmd12 were examined using qRT-PCR. (B,E) Zebrafish ZF4 cells were infected with shRNA lentivirus targeting atg9b or psmd12 for 24 h, then the mRNA levels of atg9b and psmd12 were examined using qRT-PCR. (C,F) Zebrafish ZF4 cells were infected with shRNA lentivirus targeting atg9b or psmd12 for 24 h, then the cells were moved to an incubator at 10°C for 24 h, then cell viability was examined using trypan blue staining. All experiments were performed in triplicate. n = 3, two tailed t-test, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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