ZFIN Image: Han et al., 2020, FIGURE 5

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Figure Caption

FIGURE 5

Roles of atg9b and psmd12 in cold tolerance in zebrafish cells. (A,D) zebrafish ZF4 cells were treated with 5 μM radicicol for indicated times, then the mRNA levels of atg9b and psmd12 were examined using qRT-PCR. (B,E) Zebrafish ZF4 cells were infected with shRNA lentivirus targeting atg9b or psmd12 for 24 h, then the mRNA levels of atg9b and psmd12 were examined using qRT-PCR. (C,F) Zebrafish ZF4 cells were infected with shRNA lentivirus targeting atg9b or psmd12 for 24 h, then the cells were moved to an incubator at 10°C for 24 h, then cell viability was examined using trypan blue staining. All experiments were performed in triplicate. n = 3, two tailed t-test, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

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