FIGURE

FIGURE 4

ID

ZDB-FIG-201202-4

Publication

Wang et al., 2020 - Coding Variants Coupled With Rapid Modeling in Zebrafish Implicate Dynein Genes, dnaaf1 and zmynd10, as Adolescent Idiopathic Scoliosis Candidate Genes

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FIGURE 4

CRISPR/Cas9 knockout of candidate genes in zebrafish recapitulate the scoliosis phenotype. (A) Representative phenotypes of zebrafish larvae 3 dpf (days postfertilization) that were injected with multiplexed CRISPR/Cas9 reagents to target mutagenesis to candidate adolescent idiopathic scoliosis (AIS) associated genes. (B) Histograms representing the total percentage of curve body phenotype assessed at 3 and 5 dpf after injection is shown in the bar graph with red color. The number of larval fish assessed for each targeted gene is displayed in each set of bars. (C) Bright-field images (left) and Alizarin red/Alcian Blue stained (right) zebrafish (>30 dpf). All scale bars: 1 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnaaf1^{zf3430/zf3430} dnah1^{zf3433/zf3433} dnali1^{zf3432/zf3432} tns1a^{zf3434/zf3434} zmynd10^{zf3431/zf3431}
Observed In:	vertebral column whole organism axis
Stage Range:	Protruding-mouth to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol