ZFIN Image: Wang et al., 2020, FIGURE 4

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Figure Caption

FIGURE 4

CRISPR/Cas9 knockout of candidate genes in zebrafish recapitulate the scoliosis phenotype. (A) Representative phenotypes of zebrafish larvae 3 dpf (days postfertilization) that were injected with multiplexed CRISPR/Cas9 reagents to target mutagenesis to candidate adolescent idiopathic scoliosis (AIS) associated genes. (B) Histograms representing the total percentage of curve body phenotype assessed at 3 and 5 dpf after injection is shown in the bar graph with red color. The number of larval fish assessed for each targeted gene is displayed in each set of bars. (C) Bright-field images (left) and Alizarin red/Alcian Blue stained (right) zebrafish (>30 dpf). All scale bars: 1 mm.

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Phenotype details

Acknowledgments

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