FIGURE

Fig 5

ID

ZDB-FIG-201121-24

Publication

Su�rez et al., 2020 - Identification of a non-classical three-dimensional nuclear localization signal in the intestinal fatty acid binding protein

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Fig 5

Wild-type and mutant drFabp2 ligand-dependent activation of nuclear traslocation.

A) Superimposed structures of the α-helical regions of mFABP4 bound to linolenic acid (mFABP4-LA, PDB ID: 2Q9S), mFABP4 bound to oleic acid (mFABP4-OA, PDB ID: 1LID) and hFABP2 bound to oleic acid (hFABP2-OA, PDB ID: 2MO5) α-helical region. The side chain of the Phe residue implicated in ligand-dependent response to ligands is shown for FABP4 bound to linolenic acid (blue), mFABP4 bound to oleic acid (green) and hFABP2 bound to oleic acid (red). B) Graphical representation of nuclear/cytoplasmic fluorescence intensity relationship (mean ± SD) of wild- type and ¹⁷A²⁸A²⁹A drFabp2-EGFP in Caco-2 cells cultured in media without FBS (-FBS) or in media without FBS and with oleic acid (-FBS/+OA). Statistical significance is indicated, **** p < 0.0001, n.s. not significant. C) Representative single focal planes of Caco-2 cells transfected with wild- type or ¹⁷A²⁸A²⁹A drFabp2-EGFP (green) cultured in media without FBS (-FBS) or in media without FBS and with oleic acid (-FBS/+OA). Nuclei counterstaining with DAPI is shown in blue. Bar: 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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