ZFIN Figure: Whittle et al., 2020, Figure 6

Figure 6

Slow skeletal muscle disorganization of <italic>smyhc1</italic> mutants

A

Confocal fluorescence images of slow skeletal muscle of 1 dpf larvae, stained with phalloidin‐rhodamine to detect actin. Puncta of filamentous actin are indicated with white arrowheads, and misshapen myofibers are indicated with white arrows and outlined.

B

Confocal microscope fluorescence images of slow skeletal muscle of 3 dpf larvae, stained with phalloidin‐rhodamine. Puncta of filamentous actin are indicated with white arrowheads, distorted myofibers are indicated with arrows and outlined, and frayed myofibers are indicated with yellow arrowhead.

C

Confocal fluorescence images of slow skeletal muscle of 1 dpf larvae, stained with phalloidin‐rhodamine (red), anti‐α‐actinin antibodies (green), and DAPI (blue).

D

Confocal fluorescence images of slow skeletal muscle of 3 dpf larvae, stained with phalloidin‐rhodamine (red), anti‐α‐actinin antibodies (green), and DAPI (blue). Bundle of actin ringed with α‐actinin indicated with white‐bordered black arrowhead.

E

Myoseptal intervals of slow skeletal muscle at 1 dpf in smyhc1^+/+ (n = 54), smyhc1^−/− (n = 31), smyhc1^R673H/+ (n = 45), and smyhc1^R673H/R673H (n = 49). Distance between myosepta was measured perpendicular to rostral‐caudal body axis at defined mid‐body regions in phalloidin stained slow skeletal muscle fluorescence images. The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range.

F

Myoseptal intervals of slow skeletal muscle at 3 dpf in smyhc1^+/+ (n = 51), smyhc1^−/− (n = 32), smyhc1^R673H/+ (n = 64), and smyhc1^R673H/R673H (n = 38). The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range.

G

Z‐disk intervals (sarcomere length) of slow skeletal muscle at 1 dpf in smyhc1^+/+ (n = 380), smyhc1^R673H/+ (n = 293), and smyhc1^R673H/R673H (n = 268). Distance between z‐disks was measured in anti‐α‐actinin stained slow skeletal muscle fluorescence images. The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range.

H

Z‐disk intervals (sarcomere length) of slow skeletal muscle at 3 dpf in smyhc1^+/+ (n = 249), smyhc1^−/− (n = 278), smyhc1^R673H/+ (n = 246), and smyhc1^R673H/R673H (n = 252). The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range.

Data Information: Wilcoxon rank‐sum test used to calculate significance. P‐values comparing each group displayed above data. Scale bars represent a length of 50 μm.

Expression Data

Antibody:	Ab1-actn
Fish:	WT smyhc1^{stl582/stl582} smyhc1^{stl584/stl584} smyhc1^stl584/+
Anatomical Term:	slow muscle cell Z disc
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smyhc1^{stl582/stl582} smyhc1^{stl584/stl584} smyhc1^stl584/+
Observed In:	myotome slow muscle cell slow muscle cell actin filament slow muscle cell Z disc somite
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.