Figure 6

Confocal microscope fluorescence images of slow skeletal muscle of 3 dpf larvae, stained with phalloidin‐rhodamine. Puncta of filamentous actin are indicated with white arrowheads, distorted myofibers are indicated with arrows and outlined, and frayed myofibers are indicated with yellow arrowhead.

Confocal fluorescence images of slow skeletal muscle of 1 dpf larvae, stained with phalloidin‐rhodamine (red), anti‐α‐actinin antibodies (green), and DAPI (blue).

Confocal fluorescence images of slow skeletal muscle of 3 dpf larvae, stained with phalloidin‐rhodamine (red), anti‐α‐actinin antibodies (green), and DAPI (blue). Bundle of actin ringed with α‐actinin indicated with white‐bordered black arrowhead.

Myoseptal intervals of slow skeletal muscle at 1 dpf in smyhc1^+/+ (n = 54), smyhc1^−/− (n = 31), smyhc1^R673H/+ (n = 45), and smyhc1^R673H/R673H (n = 49). Distance between myosepta was measured perpendicular to rostral‐caudal body axis at defined mid‐body regions in phalloidin stained slow skeletal muscle fluorescence images. The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range.

Myoseptal intervals of slow skeletal muscle at 3 dpf in smyhc1^+/+ (n = 51), smyhc1^−/− (n = 32), smyhc1^R673H/+ (n = 64), and smyhc1^R673H/R673H (n = 38). The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range.

Z‐disk intervals (sarcomere length) of slow skeletal muscle at 1 dpf in smyhc1^+/+ (n = 380), smyhc1^R673H/+ (n = 293), and smyhc1^R673H/R673H (n = 268). Distance between z‐disks was measured in anti‐α‐actinin stained slow skeletal muscle fluorescence images. The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range.

Z‐disk intervals (sarcomere length) of slow skeletal muscle at 3 dpf in smyhc1^+/+ (n = 249), smyhc1^−/− (n = 278), smyhc1^R673H/+ (n = 246), and smyhc1^R673H/R673H (n = 252). The central bands of the boxplots represent the median, the boxes of the boxplots represent the interquartile range (between the first and third quartile), and the whiskers represent the minimum and maximum values, up to 1.5 times the interquartile range. Outliers displayed are outside of this range.