FIGURE

Fig. 5

ID

ZDB-FIG-201027-5

Publication

Barske et al., 2020 - Evolution of vertebrate gill covers via shifts in an ancient Pou3f3 enhancer

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Fig. 5

Misexpression of pou3f3b in posterior arch mesenchyme induces ectopic posterior-directed cartilages. Cartilage (blue) and bone (red) staining reveals ectopic cartilaginous projections in the third and fourth arches (Insets) of fli1a:Gal4VP16; UAS:pou3f3b (A) and kat6a−/− (B) larvae. The hyoid skeleton was reduced in fli1a:Gal4VP16; UAS:pou3f3b embryos (arrows in A) and homeotically transformed in kat6a mutants (arrowheads in B). (C and E) In situ hybridization for pou3f3b (pink) relative to the pan-mesenchyme marker dlx2a (green) shows ectopic expression of pou3f3b in neural crest-derived mesenchyme (primarily arches 1 to 4) in fli1a:Gal4VP16; UAS:pou3f3b embryos and in the dorsal third and fourth arches (double arrows) of kat6a mutants. (D and F) In situ hybridizations show that the PEM marker shha remains confined to the hyoid arch (arrow) in fli1a:Gal4VP16; UAS:pou3f3b and kat6a−/− embryos. In situ images are maximum-intensity projections. (Scale bars: A and B, 50 μm; C–F, 20 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA