Figure 8—figure supplement 1.

Tectal cell ablation controls and enucleation experiments.

(A–C) In our free-swimming prey capture assay, 'sham' ablated larvae (gray), in which non-responsive tectal cells have been removed, did not behave differently from untreated ('ctrl', green), or agarose-embedded and released ('embed', purple) larvae, in terms of time spent in prey capture (A), initial J-turn kinematics (B), or direction selectivity index (C). (D) Cluster color-coded functional cell responses in both anterior tecta for the experiment shown in Figure 8A (arrow heads highlighting cells selected for ablations). Midline is indicated by a dashed white line. Note the tectal cell responses in the left, non-stimulated tectum. (E) Enucleated larvae expressing elavl3:nls-GCaMP6s were functionally imaged as described, and the functional cluster distribution of tectal cells was compared to untreated fish. Error bars are SEM. Functional cluster colors are described on the right. Scale bar in (D): 20 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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