FIGURE

Fig. 5

ID

ZDB-FIG-201003-129

Publication

Tsukiyama et al., 2020 - A phospho-switch controls RNF43-mediated degradation of Wnt receptors to suppress tumorigenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Cooperation of mutant RNF43 with active Ras establishes Wnt-Ras-p53 axis.

a Induction of p53 and p21 protein was examined by immunoblotting (IB) with Eto treatment. Expression of p53 and p21 in empty vector-transfected NC cells with DMSO treatment was set to 1. Bar graphs and error bars represent mean ± sd. Red circles indicate individual values of each sample. Asterisks indicate significant differences from NC cells stimulated with Eto (P < 0.05, one-way ANOVA, n = 3 biological replicates). ND indicates no significant difference. b, c Prognosis of patients with colorectal tumour that carry genetic mutations in RNF43 with or without KRAS (b) or in RNF43 with or without TP53 (c) is shown. Sample number and P value determined by log-rank test (b, c) with Holm adjustment for multiple comparisons (b) are indicated in each graph. d Schematic of biological role of RNF43 oncogenic mutations in multi-step tumorigenesis. Oncogenic RNF43 mutants that promote Wnt signalling and inhibit the p53 pathway cooperate with activating Ras mutations to complete all the steps of multi-step colorectal tumorigenesis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.