FIGURE

Figure 6—figure supplement 2.

ID

ZDB-FIG-200907-27

Publication

Wertman et al., 2020 - The identification of dual protective agents against cisplatin-induced oto-and nephrotoxicity using the zebrafish model

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Figure 6—figure supplement 2.

Representative flow plots and gating strategies for the detection of late-stage apoptosis and cell death in SK-N-AS neuroblastoma (NBL) cells.

SK-N-AS neuroblastoma (NBL) cells were treated with either vehicle control or either 0.03 mM dopamine or 0.03 mM L-mimosine for 12 hr, were rinsed, then treated with 0.01 mM cisplatin for 48 hrs. Cells were collected, rinsed, and prepared for PE-conjugated Annexin-V/SYTOXBlue flow cytometry. Data were analyzed using BD FACSDiva Software. (A–D) Representative flow plots demonstrating the four quadrants. LL=Annexin-/SYTOX-, LR=Annexin+/SYTOX-, UL=Annexin-/SYTOX+, UR=Annexin+/SYTOX+. (A) Control untreated cells, (B) Cisplatin only, (C) dopamine + cisplatin, (D) L-mimosine + cisplatin. N=4. (E) General gating strategy to identify living and dead cells in sample and to eliminate cell debris. (F) Unstained control cells. (G) Doublet discrimination strategy. (H) Unstained control cells. (I) Gating strategy to identify SYTOX+ dead cells. Representative plots from heat-killed SYTOX+ control cells. (J) Gating strategy to identify Annexin+ cells. Representative flow plot from cells treated with Camptothecin to induce apoptosis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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