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A reciprocal regulatory loop for miR-430 in the clearance of maternal transcript. (A) The transcripts of Nanog, Dicer1, Dgcr8, and AGOs were maternally provided. (B) The maternally provided transcripts Nanog, Dicer1, Dgcr8, and AGOs were significantly increased in the miR-430–/– mutants and down-regulated in the rescued embryos at shield stage. (C) WISH detection of Dicer1 transcripts in the WT and miR-430–/– mutants. The Dicer1 transcripts were maternally provided and degraded at shield stage in the WT but not miR-430-deficient embryos. (D) Repression of Dicer1 by miR-430 thorough miR-430 binding sites in their 3′UTR. Two miR-430 binding sites (GCACTT) were identified in the 3′UTR of Dicer1. GFP sensors with Dicer-3′UTR-WT and Dicer-3′UTR-Mut (two-nucleotide mutations in the miR-430 binding sites) were injected into embryos and fluorescence was examined at 6 hpf. (E) A proposed reciprocal regulatory loop between maternally provided transcripts and miR-430.
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