FIGURE

Fig 2

ID

ZDB-FIG-200729-2

Publication

Osman et al., 2020 - Mycobacterium marinum phthiocerol dimycocerosates enhance macrophage phagosomal permeabilization and membrane damage

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Fig 2

PDIM transport deficiency reduces <italic>M</italic>. <italic>marinum</italic>-induced phagosomal membrane damage.

(A) Maximum intensity projections of confocal micrographs showing galectin-8 labeling of PMA-differentiated THP-1 cells infected with tdTomato-expressing wildtype or mutant M. marinum strains 22 hours post infection. Arrows indicate co-localization of Galectin-8 (green) and M. marinum (magenta) fluorescence. Bottom panels show close-up views of the corresponding boxed regions in the top panels. Scale bars, 50μm. (B) Percent of infected cells that have mycobacterial foci labeled with galectin-8. Symbols represent individual imaging fields. Horizontal lines depict mean values. The respective means and 95% confidence intervals for wildtype, ΔESX-1 and ΔmmpL7 infections are 28.9 (22.0–35.8), 0.7 (0.2–1.2), and 4.7 (3.1–6.3). Statistical significance was determined using a Kruskal-Wallis nonparametric test with Dunn’s post-hoc test to control for multiple comparisons, **** p < 0.0001, * p < 0.05. Data are representative of two experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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