FIGURE

Figure 3

ID

ZDB-FIG-200725-3

Publication

Poplimont et al., 2020 - Neutrophil Swarming in Damaged Tissue Is Orchestrated by Connexins and Cooperative Calcium Alarm Signals

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Figure 3

5-LO-Associated Calcium Fluxes Are Triggered upon Contact with Necrotic Cells or Neutrophils with Ongoing Fluxes

(A) Schematic of two-photon laser wounding in the presence of propidium iodide (PI).

(B) Time-lapse, two-photon confocal projection images of a GCamp6F-expressing (white) neutrophil (indicated with an arrow) entering a contact with PI⁺ cells/tissue (red) in a Tg(lyz:GCamp6F) larva; time in relation to the first frame is indicated in seconds. Scale bar represents 10 μm.

(C) Quantification of speed (blue) and normalized GCamp6F (gray) in neutrophils before and after contact with PI⁺ tissue. GCamp6F intensity was normalized as in Figures 1D and 1E. Dotted red line indicates time of contact. Pooled cell data from n = 23 cells in 7 larvae and 4 experiments are shown.

(D) Examples of cell contacts transmitting calcium fluxes. Each case is represented by time-lapse images of a non-calcium-fluxing neutrophil (arrow) contacting a fluxing neutrophil. Time in minutes is indicated relative to cell-cell contact. Scale bar represents 10 μm. The quantification of neutrophil transmission of calcium fluxes is indicated in Figure 6.

Error bars represent SEM. See also Figure S2 and Video S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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