FIGURE

Figure 9

ID

ZDB-FIG-200725-28

Publication

Toms et al., 2020 - Clinical and preclinical therapeutic outcome metrics for USH2A-related disease

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Figure 9

Retinal ultrastructure of ush2a^u507 zebrafish. Transmission electron microscopy was used to examine the wt and ush2a^u507 retinal ultrastructure at 6 months post fertilization (mpf). The wt retina showed tiers of morphologically distinct cone and rod photoreceptors with overlying retinal pigment epithelium (RPE) with long apical projections that interdigitated with the photoreceptors (A). Among mostly preserved tissue, regions of rod loss could be observed in the ush2a^u507 retina (B). Presumptive lysosomal structures (blue arrows) were noted at the inner and outer segment boundary (D, E) and at the synapses (G) of the ush2a^u507 photoreceptors at 6 mpf. Ribbon synapses are indicated by ^*. These vesicles were not observed in wt photoreceptors (C, F). IS, inner segment; OS, outer segment; IS, inner segment; OS, outer segment; RPE, retinal pigment epithelium; BM, Bruch’s membrane; N, nucleus; P, phagosome; M, melanosome. Scale bars = 5 μm (A, B, F, G) and 500 nm (C–E).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ush2a^{zf3378/zf3378} (AB)
Observed In:	photoreceptor cell lysosome photoreceptor inner segment layer lysosome photoreceptor outer segment layer lysosome retinal rod cell
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.