ZFIN ID: ZDB-FIG-200619-38
Safarian et al., 2020 - Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling. Scientific Reports   10:9538 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Day 6 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 6 to Adult

Figure 2

Phenotypic characterization of panx1a−/− larvae – (a) Age-matched wild type TL and panx1a−/− larvae (6 dpf) showing regular morphology. (b) RT-qPCR analysis of pannexin expression in 6 dpf larva. (c) The panx1a expression is reduced in the adult panx1a−/− zebrafish. The expression was determined using an affinity purified rabbit anti-panx1a antibody directed against the carboxy-terminal 129 amino acids of the Panx1a protein. Detection of the PSD-95 protein in cone terminals served as an internal control. Cell nuclei were stained with DAPI. In wild type TL fish arrows indicate Panx1a immunoreactivity found in the horizontal cell layer as reported previously19,20. The immunoreactivity was significantly reduced in panx1a−/− fish. Residual staining was overlapping with DAPI stained nuclei and considered as unspecific. Abbreviations: PL, photoreceptor layer; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar 100 µm. n.d., Not detected. Significance: ***p-value < 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
panx1a Ab1-panx1a TL standard conditions Adult horizontal cell IHC
Ab1-panx1a panx1ayku1/yku1 standard conditions Adult horizontal cell IHC
Ab1-panx1a Adult whole organism IHC
panx1b TL standard conditions Day 6 whole organism RTPCR
panx1ayku1/yku1 standard conditions Day 6 whole organism RTPCR
panx2 TL standard conditions Day 6 Not Detected whole organism RTPCR
panx1ayku1/yku1 standard conditions Day 6 Not Detected whole organism RTPCR
panx3 TL standard conditions Day 6 whole organism RTPCR
panx1ayku1/yku1 standard conditions Day 6 whole organism RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-dlg4 IHC TL standard conditions Adult retinal cone cell
IHC panx1ayku1/yku1 standard conditions Adult retinal cone cell
Phenotype Details
Fish Conditions Stage Phenotype
panx1ayku1/yku1 standard conditions Day 6 retina morphology, normal
Day 6 whole organism morphology, normal
Adult horizontal cell panx1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.