PUBLICATION

Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling

Authors

Safarian, N., Whyte-Fagundes, P., Zoidl, C., Grigull, J., Zoidl, G.

ID

ZDB-PUB-200615-5

Date

2020

Source

Scientific Reports 10: 9538 (Journal)

Registered Authors

Zoidl, Georg

Keywords

none

Datasets

GEO:GSE147068

MeSH Terms

Superior Colliculi/drug effects
Superior Colliculi/metabolism
Receptors, Dopamine D2/metabolism
Animals
Zebrafish/metabolism*
Haloperidol/pharmacology
Retina/drug effects
Retina/metabolism
Dopamine D2 Receptor Antagonists/pharmacology
Dopamine/metabolism*
Swimming/physiology
Connexins/metabolism*
Neurons/drug effects
Neurons/metabolism*
Vision, Ocular/drug effects
Vision, Ocular/physiology*
Signal Transduction/drug effects
Signal Transduction/physiology*
Larva/drug effects
Larva/metabolism
Zebrafish Proteins/metabolism*

(all 21)

PubMed

32533080 Full text @ Sci. Rep.

Abstract

Pannexin 1 (Panx1) forms ATP-permeable membrane channels that play roles in the nervous system. The analysis of roles in both standard and pathological conditions benefits from a model organism with rapid development and early onset of behaviors. Such a model was developed by ablating the zebrafish panx1a gene using TALEN technology. Here, RNA-seq analysis of 6 days post fertilization larvae were confirmed by Real-Time PCR and paired with testing visual-motor behavior and in vivo electrophysiology. Results demonstrated that loss of panx1a specifically affected the expression of gene classes representing the development of the visual system and visual processing. Abnormal swimming behavior in the dark and the expression regulation of pre-and postsynaptic biomarkers suggested changes in dopaminergic signaling. Indeed, altered visuomotor behavior in the absence of functional Panx1a was evoked through D1/D2-like receptor agonist treatment and rescued with the D2-like receptor antagonist Haloperidol. Local field potentials recorded from superficial areas of the optic tectum receiving input from the retina confirmed abnormal responses to visual stimuli, which resembled treatments with a dopamine receptor agonist or pharmacological blocking of Panx1a. We conclude that Panx1a functions are relevant at a time point when neuronal networks supporting visual-motor functions undergo modifications preparing for complex behaviors of freely swimming fish.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
panx1a^yku1/yku1	standard conditions	Day 6	amacrine cell th2 expression increased amount, abnormal	Fig. 6
panx1a^yku1/yku1	standard conditions	Day 6	retina morphology, normal	Figure 2
panx1a^yku1/yku1	standard conditions	Day 6	visual behavior process quality, abnormal	Fig. 5
panx1a^yku1/yku1	standard conditions	Day 6	whole organism adora1b expression decreased amount, abnormal	Fig. 3
panx1a^yku1/yku1	standard conditions	Day 6	whole organism cacna1da expression decreased amount, abnormal	Fig. 3
panx1a^yku1/yku1	standard conditions	Day 6	whole organism gna11b expression increased amount, abnormal	Fig. 3
panx1a^yku1/yku1	standard conditions	Day 6	whole organism gnat1 expression increased amount, abnormal	Fig. 3
panx1a^yku1/yku1	standard conditions	Day 6	whole organism gnat2 expression increased amount, abnormal	Fig. 3
panx1a^yku1/yku1	standard conditions	Day 6	whole organism grk7b expression decreased amount, abnormal	Fig. 3
panx1a^yku1/yku1	standard conditions	Day 6	whole organism guca1c expression increased amount, abnormal	Fig. 3

1 - 10 of 40

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
yku1		Small Deletion	panx1a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
panx1a	TALEN2-panx1a	TALEN

Fish

Fish
panx1a^yku1/yku1
TL

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-panx1a	polyclonal	panx1a	IgG	Rabbit
Ab3-dlg4	monoclonal		IgG1	Mouse

Orthology

Engineered Foreign Genes

Mapping

Safarian et al., 2020 ZDB-PUB-200615-5 PMID:32533080

Visuomotor deficiency in panx1a knockout zebrafish is linked to dopaminergic signaling

Safarian et al., 2020

ZDB-PUB-200615-5
PMID:32533080