FIGURE

Fig. 5.

ID

ZDB-FIG-200608-19

Publication

Ates et al., 2020 - Deficiency in the endocytic adaptor proteins PHETA1/2 impair renal and craniofacial development

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Fig. 5.

Loss of pheta1/2 disrupts chondrocyte maturation. (A) Ceratohyal cartilage, stained with Alcian Blue in flat-mount preparation. Scale bars: 50 µm. (B) Chondrocyte morphology analysis in ceratohyal cartilage (within a 200 µm² area). (C) Top row: Z-projection ventral view of larvae immunostained for Col2 (green). Nuclei are labeled with DAPI (blue). Scale bars: 100 μm. Middle row: higher-magnification Z-projection images of corresponding ceratohyal cartilage. Scale bars: 25 μm. Bottom row: higher-magnification single optical section images of corresponding ceratohyal cartilage, depicting extracellular secretion of type II collagen. Scale bars: 25 µm. (D,E) Quantification of mean fluorescence intensity in the ceratohyal (D) and Meckel's cartilage (E) in 5 dpf larvae. *P<0.05, ***P<0.001, ****P<0.0001.

Expression Data

Antibody:	Ab1-col2a
Fish:	WT pheta1^vt2/vt2 pheta2^vt3/vt3 pheta1^vt2/vt2; pheta2^vt3/vt3
Anatomical Terms:	ceratohyal cartilage Meckel's cartilage palatoquadrate cartilage
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pheta1^vt2/vt2 pheta2^vt3/vt3 pheta1^vt2/vt2; pheta2^vt3/vt3
Observed In:	ceratohyal cartilage chondrocyte axis elongation Meckel's cartilage
Stage Range:	Day 5 to Day 6

Phenotype Detail

Acknowledgments

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