Fig. 2

(A) Cre recombination was sparsely induced in 3-mpf her4.1:ERT2CreERT2;ubi:Switch double transgenic adults by 4-OHT, resulting in mCherry expression in recombined NSCs and their progeny. Analyzed time points (arrows) span between 6 and 507 dpi. (B) Dorsal view of a representative pallium showing sparsely induced cells at 6 dpi (dotted area to the pallial Dm territory of interest; fig. S1E). Boxed areas are magnified to illustrate the different cell types traced (yellow arrows). Proliferating progenitors were labeled by a 24-hour 5-bromo-2′-deoxyuridine (BrdU) pulse. NSCs are Gs⁺ and Sox2⁺, NPs are Sox2⁺ only. In contrast to aNSCs and aNPs, qNSCs and qNPs did not incorporate BrdU during the pulse. (C) Cell type composition of the mCherry⁺ population at 6 dpi. n = 6 brains. (D) Example of clustering at 183 dpi. Left: Dorsal view of a 3D reconstruction of a hemisphere. The spots registering the coordinates of traced cells (red) are shown (white dots). Right: 2D map of the highest probability clonal partition identified by the statistical inference. Axes correspond to the x and y coordinates (in μm) of the 2D projection of the cell centers. Cells belonging to the same clone are embedded in the same shaded area. (E) Average number of traced (mCherry⁺) Gs⁺/Sox2⁺ NSCs per hemisphere across all time points analyzed. One-way analysis of variance (ANOVA): F_(8,37) = 1, P = 0.45; all pairwise comparisons: least significant difference (LSD) test followed by Holm’s adjustment. Error bars, 95% confidence interval (CI). (F) Average number of traced (mCherry⁺) Sox2⁺ cells per hemisphere across all time points analyzed. One-way ANOVA: F_(8,37) = 1.65, P = 0.14; all pairwise comparisons: LSD test followed by Holm’s adjustment. Error bars, 95% CI. (E and F) n = 6, 3, 3, 3, 4, 6, 7, 8, and 6 brains at 6, 18, 30, 64, 91, 125, 183, 307, and 507 dpi, respectively.