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<italic>her4.1</italic>:dRFP and <italic>gfap</italic>:nlsGFP expressions characterize the same subpopulation of Sox2-expressing pallial progenitors.(A) Three-dimensional (3D) reconstruction (dorsal view) of the pallium of a her4.1:dRFP/gfap:nlsGFP double transgenic adult immunostained for GFP, dRFP, Sox2, and the proliferation marker proliferating cell nuclear antigen (Pcna) [labeling the same cells as the proliferation marker minichromosome maintenance 5 (21)]. The lateral and the medial pallial domains (Dl and Dm) are highlighted with dotted lines. Close-ups of the boxed area with different combinations of channels illustrating the four Sox2+ progenitor subtypes that make up the pallial germinal zone are shown: quiescent NSCs (qNSCs; blue arrow), activated NSCs (aNSCs; yellow arrow), qNPs (white arrow), and aNPs (pink arrow). (B) Distribution of gfap+ and her4.1+ NSCs, alone and in combination, among Sox2+ cells. (C) Respective distributions of her4.1+ and gfap+ cells among the gfap+ and the her4.1+ populations. Paired t test: P = 0.34. (D) Relative proportions of quiescent (Pcna−) and proliferating (Pcna+) gfap+ NSCs, her4.1+ NSCs, and NPs (Sox2+). The data were analyzed using a repeated measures mixed model. Overall tests: P < 0.001; pairwise comparisons: ***P < 0.001 after Holm’s adjustment. (E) Distribution of gfap+ and her4.1+ NSCs together with Sox2+ NPs among proliferating (Pcna+) progenitors. (F) Summary of markers characterizing Dm pallial progenitors. (B to E) n = 7 brains were analyzed. Error bars, SEM.
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