Fig. 3

Ezetimibe treatment results in reduced neutral lipid accumulation and reduced infection burden. a. Comparison of integrated density of filipin fluorescence in the tail region of animals treated with vehicle alone (0.5% DMSO) or 1 μM ezetimibe. Data were collected at 3 and 6 days post-treatment (dpt). Student’s t-test *p = 0.018, ****p<0.0001 b. Comparison of integrated density of Nile red fluorescence in the tail region of animals treated with vehicle alone (0.5% DMSO) or 1 μM ezetimibe. Data were collected at 3 and 6 dpt. Student’s t-test ****p<0.0001 c. Animals representing the median values from comparison groups in (a) and (b). Bar = 500μm. d. Four-day time course of bacterial growth in vitro. Culture was maintained in either vehicle alone (0.5% DMSO) or 1 μM ezetimibe. No significant differences in bacterial growth were observed, Pearson R-squared = 0.99. e. Representative images of larval tail fins 2dpi in the absence or presence of ezetimibe treatment. Arrows indicate location of infection foci with (DMSO) or without (ezetimibe) neutral lipid accumulation. Bar = 100μm f. Comparison of infection burden at 24 hpi, 3 dpi, and 6 dpi in animals treated with vehicle alone (DMSO), 1 μM and 5 μM ezetimibe. No statistically significant differences were observed between groups at 24 hpi and 3dpi. One- way ANOVA with Tukey’s multiple comparisons ****p<0.0001. Representative of four independent experiments. g. Animals representing the median values from 6 dpi comparison groups in (f).