ZFIN ID: ZDB-FIG-200515-22
Wang et al., 2020 - The micropeptide LEMP plays an evolutionarily conserved role in myogenesis. Cell Death & Disease   11:357 Full text @ Cell Death Dis.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 4 a (Left): Representative images of H&E-staining of TA muscle cross-sections at day 5 and day 7 post injury from WT and LEMP KO mice. Scale bar, 50 μm. (Right): Statistics on the number of regeneration fibers per unit area. Error bars, standard deviations (n = 10). Statistical analysis was performed using Student’s t test. ***P < 0.001. b MHC immunostaining of satellite cells isolated from WT and LEMP KO mice. MHC: green; DAPI: red. c Quantitation of fusion in WT and LEMP KO satellite cells. Scale bar, 100 μm. Error bars, standard deviations (n = 3). Statistical analysis was performed using Student‘s t test. **P < 0.01, *P < 0.05, ns: not significant. d RT-qPCRs to examine MHC mRNA expression level in satellite cells isolated from WT and LEMP KO mice. The bars show RNA levels relative to GAPDH. Error bars, standard deviations (n = 3). Statistical analysis was performed using Student’s t test. **P < 0.01.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-lnc.lemp standard conditions Prim-5 skeletal muscle disorganized, abnormal
Prim-5 skeletal muscle fiber development disrupted, abnormal
Long-pec skeletal muscle disorganized, abnormal
Long-pec skeletal muscle fiber development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Death & Disease for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Death Dis.