Fig. 4 a (Left): Representative images of H&E-staining of TA muscle cross-sections at day 5 and day 7 post injury from WT and LEMP KO mice. Scale bar, 50??m. (Right): Statistics on the number of regeneration fibers per unit area. Error bars, standard deviations (n?=?10). Statistical analysis was performed using Student?s t test. ***P?0.001. b MHC immunostaining of satellite cells isolated from WT and LEMP KO mice. MHC: green; DAPI: red. c Quantitation of fusion in WT and LEMP KO satellite cells. Scale bar, 100??m. Error bars, standard deviations (n?=?3). Statistical analysis was performed using Student?s t test. **P?0.01, *P?0.05, ns: not significant. d RT-qPCRs to examine MHC mRNA expression level in satellite cells isolated from WT and LEMP KO mice. The bars show RNA levels relative to GAPDH. Error bars, standard deviations (n?=?3). Statistical analysis was performed using Student?s t test. **P?0.01.
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