FIGURE

Fig. 5

ID

ZDB-FIG-200501-30

Publication

Kobayashi-Sun et al., 2020 - Uptake of osteoblast-derived extracellular vesicles promotes the differentiation of osteoclasts in the zebrafish scale

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Fig. 5

Isolation of OB-derived EVs.

a Representative flow cytometric analysis of cells in fractured scales at 1 day post-fracture (dpf) from an osterix:mCherry single-transgenic zebrafish. Gated regions in the left panel indicate the osterix:mCherry⁺ Hoecht 33342^high (mCh⁺ Hoe^high) cell fraction and mCh⁺ Hoe^low EV fraction. mCh⁺ Hoe^high cells and mCh⁺ Hoe^low EVs are displayed in a forward scatter (FSC) vs. side scatter (SSC) dot plot (middle and right panels, respectively). b Representative electron microscopic images of isolated large and small EVs. c Representative image of isolated EVs negatively stained. Arrows indicate an EV. d Percent size distribution of EVs (n = 223). e Absolute number of mCh⁺ Hoe^low EVs in an intact and fractured scale at 1 dpf. **p < 0.01 (n = 9 for each group). Bars, 1 μm b; 2 μm c. Experiments were performed twice with two biological replicates a–d and nine biological replicates e in each group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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