ZFIN ID: ZDB-FIG-200427-2
Wu et al., 2020 - Role of the ADCY9 gene in cardiac abnormalities of the Rubinstein-Taybi syndrome. Orphanet journal of rare diseases   15:101 Full text @ Orphanet J Rare Dis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 2

Effects of adcy9 knock down on macrophage migration. a-f Representative bright field and fluorescent images of TG (zlyz: EGFP) larvae at 6 days postfertilization (dpf). Control MO injected fish show the normal distribution of labeled cells (b and c). Compared with control fish, embryos injected with adcy9-e3i3-MO present potent macrophage migration in heart (e and f, red circle area). g-k Quantification of the macrophage number at heart shows a 13.3-fold increased in adcy9 morphants. Columns, mean; bars, SEM (n = 10; Student’s t test; ***P < 0.0001;). dpf, days post fertilization. l Endogenous efnb2a, COX2, mmp9, ptp-rb and pik3r2 in control and adcy9 morphants assessed by qRT-PCR (n = 6–10 individual embryos). ns, not significant

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mmp9 AB control Protruding-mouth whole organism RTPCR
AB + MO1-adcy9 standard conditions Protruding-mouth whole organism RTPCR
pik3r2 AB control Protruding-mouth whole organism RTPCR
AB + MO1-adcy9 standard conditions Protruding-mouth whole organism RTPCR
ptprb AB control Protruding-mouth whole organism RTPCR
AB + MO1-adcy9 standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-adcy9 standard conditions Protruding-mouth whole organism mmp9 expression increased amount, abnormal
rj1Tg + MO1-adcy9 standard conditions Day 6 heart macrophage chemotaxis increased occurrence, abnormal
Acknowledgments:
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