FIGURE

Figure 5.

ID
ZDB-FIG-200421-67
Publication
Scaletti et al., 2020 - MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool
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Figure 5.

Activity with N6-methyl-dATP is evolutionary conserved among vertebrates. MutT homologues (MTH1 and NUDT1) from different animal species as well as E. coli MutT and NUDT1 from the plant A. thaliana were screened for hydrolysis activity with N6-methyl-dATP. Enzyme (1.25 nm) was incubated with 50 μm N6-methyl-dATP in MTH1 reaction buffer (pH 7.5) with 0.4 units/ml of PPase for 20 min at 22 °C in triplicates. Pi was detected using Biomol Green (Enzo Life Sciences). Absorbance at 630 nm was read after 20 min. A Pi standard curve was included on the plate and used to convert the assay signal to produced PPi. Data are shown as hydrolyzed N6-methyl-dATP (μm) divided by concentration of NUDT1 enzyme (μm) per second. The graph shows the mean ± S.D. from an experiment performed in triplicate.
Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.
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