FIGURE

Fig. 4

ID

ZDB-FIG-200416-3

Publication

Wu et al., 2020 - Disruption of dmrt1 rescues the all-male phenotype of cyp19a1a mutant in zebrafish - A Novel insight into the roles of aromatase/estrogens in gonadal differentiation and early folliculogenesis

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Fig. 4

Disrupting all the estrogen receptors in dmrt1 mutants. (A) Sex ratios at 75 dpf in different mutation combinations of five genes (dmrt1, cyp19a1a, esr1, esr2a and esr2b). The data were pooled from fish of five independent breedings to enlarge the sample size. (B) Representative histological sections corresponding to different gonadal types, including the ovaries in heterozygous control, quadruple knockout (dmrt1−/−; esr1−/−;esr2a−/−;esr2b−/−) and quintuple knockout (dmrt1−/−;cyp19a1a−/−;esr1−/−;esr2a−/−; esr2b−/−) at 75 dpf (n=9-43 for each genotype). Arrows indicate atresic/degenerating follicles. PV, pre-vitellogenic; EV, early vitellogenic.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dmrt1^umo15/umo15 (AB) esr2b^umo13/umo13 cyp19a1a^umo5/+ (AB) cyp19a1a^umo5/+; dmrt1^umo15/+; esr1^umo10/umo10; esr2a^umo12/umo12; esr2b^umo13/+ cyp19a1a^umo5/+; dmrt1^umo15/+; esr1^umo10/umo10; esr2a^umo12/umo12; esr2b^umo13/umo13 cyp19a1a^umo5/+; dmrt1^umo15/umo15; esr1^umo10/+; esr2a^umo12/+; esr2b^umo13/umo13 cyp19a1a^umo5/+; dmrt1^umo15/umo15; esr1^umo10/umo10; esr2a^umo12/umo12; esr2b^umo13/+ cyp19a1a^umo5/+; dmrt1^umo15/umo15; esr1^umo10/umo10; esr2a^umo12/umo12; esr2b^umo13/umo13 cyp19a1a^umo5/umo5; dmrt1^umo15/+; esr1^umo10/+; esr2a^umo12/+; esr2b^umo13/umo13 cyp19a1a^umo5/umo5; dmrt1^umo15/+; esr1^umo10/umo10; esr2a^umo12/umo12; esr2b^umo13/+ cyp19a1a^umo5/umo5; dmrt1^umo15/+; esr1^umo10/umo10; esr2a^umo12/umo12; esr2b^umo13/umo13 cyp19a1a^umo5/umo5; dmrt1^umo15/umo15; esr1^umo10/+; esr2a^umo12/+; esr2b^umo13/+ cyp19a1a^umo5/umo5; dmrt1^umo15/umo15; esr1^umo10/+; esr2a^umo12/+; esr2b^umo13/umo13 cyp19a1a^umo5/umo5; dmrt1^umo15/umo15; esr1^umo10/umo10; esr2a^umo12/umo12; esr2b^umo13/+ cyp19a1a^umo5/umo5; dmrt1^umo15/umo15; esr1^umo10/umo10; esr2a^umo12/umo12; esr2b^umo13/umo13
Observed In:	female organism male organism ovarian follicle
Stage:	Days 45-89

Phenotype Detail

Acknowledgments

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