Endothelial cell-specific wnk1a expression promote tumorigenesis in HCC-transgenic fish. (A–F) QPCR analysis of genes in WT, HBx, src(p53-) fish, and HBx, src(p53-) fish overexpressing wnk1a in endothelium. Two separate transgenic lines, TG1 and TG2, were studied. (G–J) Representative images of H&E-stained liver sections. Scale bar = 20 μm. (K–N) Representative images of immunohistochemical staining of liver sections for proliferating cell nuclear antigen (PCNA). Scale bar = 20 μm. The statistical significance was analyzed by unpaired Student’s t-test. **: 0.001 < p ≤ 0.01; ***: 0.0001 < p ≤ 0.001; ****: p ≤ 0.0001 compared to WT control. (O) Distribution of histopathological findings of H&E-stained liver sections as shown in panels (G–J). The statistical significance was analyzed by Chi-square test. ****: p ≤ 0.0001 compared to WT control, ####: p ≤0.0001 comparison between transgenic fish. (P) Average immunoreactive score (IRS) of proliferating cell nuclear antigen (PCNA) staining as shown in panels K-N. **: 0.001 < p ≤ 0.01; ***: 0.0001 < p ≤ 0.001; ****: p ≤ 0.0001 compared to WT control. ##: 0.001 < p ≤ 0.01 comparison between transgenic fish.
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