FIGURE

Fig 7

ID

ZDB-FIG-200412-20

Publication

Lee et al., 2020 - Chondroitin sulfate proteoglycan 4 regulates zebrafish body axis organization via Wnt/planar cell polarity pathway

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Fig 7

The phenotypes of <italic>cspg4</italic> morphants were rescued by co-injecting <italic>wnt11</italic> mRNA.

(A) Wnt11 had binding affinity with Cspg4. Wnt11-HA (indicated by arrow) was co-precipitated with Cspg4-flag. Asterisk mark (*) indicates non-specific band. (B) The abnormal body axis formation phenotype in cspg4 morphants (4ng MO-e2i2) was rescued by co-injecting 25 pg wnt11 mRNA. Yellow lines indicate the angle between anterior end and tailbud. (C) Co-injecting 25 pg wnt11 mRNA with 4 ng cspg4 morpholino rescued the short body length phenotype. (D) The angle between anterior end and tailbud was reduced in wnt11 rescue group compared to cspg4 knockdown group (control, n = 41; cspg4 knockdown, n = 89; wnt11 rescue, n = 117). (E) The body length of wnt11 rescue group at 1 dpf was significantly longer than cspg4 knockdown group (control, n = 40; cspg4 knockdown, n = 66; wnt11 rescue, n = 109). The significant difference was tested using ANOVA followed by Tukey’s multiple comparison. Different letters (a, b or c) indicate significant difference between groups (p ≤ 0.05). Data are presented as mean ± SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-cspg4
Knockdown Reagent:	MO1-cspg4
Observed In:	axis elongation whole organism anterior-posterior axis
Stage Range:	Bud to Prim-5

Phenotype Detail

Acknowledgments

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