FIGURE

Fig 2

ID

ZDB-FIG-200412-15

Publication

Lee et al., 2020 - Chondroitin sulfate proteoglycan 4 regulates zebrafish body axis organization via Wnt/planar cell polarity pathway

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Fig 2

Zebrafish embryo expressed <italic>cspg4</italic> during development.

(A) The expression of cspg4 was detected by RT-PCR from 0 to 120 hpf with actb1 as loading control. (B-H) Expression pattern of cspg4 was revealed by whole-mount in situ hybridization. At 9 (B) and 12 hpf (C), cspg4 was expressed in both the anterior and posterior end of the embryos. Lateral view (D) and dorsal view (E) of 20 hpf embryos showed that cspg4 was mainly expressed in the anterior paraxial and lateral mesoderm (arrow head). (F) Cspg4 was expressed in head, somite, ventral mesenchyme and tail at 24 hpf. Scale bar represents 100 μm. (G) Cspg4 was expressed in head mesenchyme (arrow) and otic placode (asteridsk). (H) Lateral view of 24 hpf embryo showed that cspg4 was expressed in the head mesenchyme (arrow) and pharyngeal region. (I) Cspg4 was expressed in the ventral sclerotome. (J) Cspg4 expressed in jaw, pectoral fins, and trunk at 4 dpf. (K) Ventral view of a 4 dpf larvae showed that cspg4 was expressed in pharyngeal cartilage and the pectoral fin.

Expression Data

Gene:	cspg4
Fish:	AB
Anatomical Terms:	anterior lateral mesoderm head mesenchyme inner ear paraxial mesoderm anterior side pectoral fin pharyngeal arch sclerotome trunk ventral mesenchyme whole organism whole organism anterior side whole organism posterior side
Stage Range:	1-cell to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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