Figure 9

FH moderated EMT marker expression in TNBC xenograft tumor zebrafish. 200 TGFβ1 48 h treated TNBC cells labeled with a red fluorescent dye (CM‐DiI) were injected into 2dpf normal zebrafish yolk sac. After xenograft tumor model was established, the tumor models were treated with FH‐L, FH‐M, FH‐H, CDDP, and control. The protein level of TGFβ1, E‐cadherin, Snail2, ZEB2, and TWIST1 was determined by Western blotting (A). β‐actin was as an inner control. Target protein expression was normalized against β‐actin protein expression. The mRNA expression of TGFβ1, Snail2, ZEB2, and TWIST1 was determined by RT‐qPCR, the mRNA level of β‐actin was used as an inner control, and gene‐specific mRNA expression was normalized against β‐actin expression (B). Normal represents normal wild‐type AB strain zebrafish, Mod represents the TNBC xenograft tumor model in zebrafish, CDDP represents cisplatin (15 μg/mL), FH‐L represents Fangjihuangqi Decoction (55.5 μg/mL), FH‐M represents Fangjihuangqi Decoction (111 μg/mL), and FH‐H represents Fangjihuangqi Decoction (222 μg/mL). **P < .01, *P < .05, compared with model group