Figure 8

FH affected tumor lymphangiogenesis in TNBC xenograft tumor zebrafish. 200 TGFβ1 48 h treated TNBC cells labeled with a red fluorescent dye (CM‐DiI) were injected into 2dpf normal transgenic vascular green fluorescent zebrafish yolk sac. After xenograft tumor model was established, the tumor models were treated with FH‐L, FH‐M, FH‐H, CDDP, and control. The rhodamine‐labeled dextran (TRITC‐Dextran) intravenously was injected into the zebrafish tumor models for angiography. The lymphatic vessels were observed under a laser confocal microscope (original magnification, 160×). Red arrow indicates lymphatics. The length of lymphatic vessels above the zebrafish cloaca was used to evaluate the inhibition ratio of FH on lymphatic vessels. Normal represents normal transgenic vascular green fluorescent zebrafish, Mod represents the TNBC xenograft tumor model in zebrafish, bevacizumab represents bevacizumab (250 ng/tail), FH‐L represents Fangjihuangqi Decoction (55.5 μg/mL), FH‐M represents Fangjihuangqi Decoction (111 μg/mL), and FH‐H represents Fangjihuangqi Decoction (222 μg/mL). **P < .01, *P < .05, compared with model group