Overexpression of mutant Kif1aa causes abnormal behavior and epileptiform discharges in transgenic zebrafish larvae. (A) Graphic representation of the experimental protocol used for the zebrafish studies. (B) Representative images of zebrafish larvae microinjected with A433D-kif1aa (n = 64) or wild type (WT)-kif1aa (n = 61) selected for local field recordings. The distribution of the labeled protein is shown by green fluorescence. (C) Seizure behaviors of zebrafish larvae. The sample locomotion tracking plots are shown on the top (S0, baseline activity; S1, small increase in swim activity; and S2, large increase in movement. The bar plot shows the percentage of tested larvae recorded during locomotion tracking (*p < 0.05, χ2 test). (D) Representative trace of spontaneous epileptiform activity recorded from zebrafish larvae. The top trace represents a typical epileptiform pattern observed in gap-free recordings. The bottom trace shows a high-resolution magnification of the selected epileptiform events, and the frequency spectrum corresponding to the selected local field potentials (LFPs) is shown on the right. Color scale: blue (low magnitude) to red (high magnitude). (E) Percentage of larvae with abnormal epileptiform activity after overexpression of A433D (n = 64) or WT Kif1aa (n = 61). (**p < 0.01, χ2 test).
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