FIGURE

Figure 4

ID

ZDB-FIG-200406-277

Publication

Mateus et al., 2020 - BMP Signaling Gradient Scaling in the Zebrafish Pectoral Fin

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Figure 4

Smoc1 Is Repressed by BMP Signaling

(A–C) BRE:GFP signal (green) and Smoc1 immunostaining (red) in the fin, at 48 hpf.

(D–G) Smoc1 expression pattern at 2 h post-heat-shock induction (hpHS) of a dominant-negative BMP receptor transgene (dnBmpr-GFP⁺) or in sibling controls (for heat-shock conditions, see STAR Methods), at 48 hpf. Inset, GFP immunostaining to monitor expression of transgene.

(H) Average intensities of normalized Smoc1 immunostainings and BRE:GFP signal versus relative position (ROI midline), at 48 hpf. Intensities were normalized to respective maxima.

(I) Average intensity of normalized Smoc1 immunostainings versus relative position (ROI midline) in dnBmpr-GFP⁺ transgenics and siblings control. Smoc1 intensity in dnBmpr-GFP⁺ was normalized to the control maxima. Shadowing corresponds to SEM per relative position (H, I).

Scale bars, 50 μm. n represents number of fins analyzed. Anterior, left; distal, down. BRE:GFP transgene used: BRE:eGFP (Laux et al., 2011) (A–C, H). See also Figure S3.

Expression Data

Gene:	smoc1
Fish:	AB
Anatomical Term:	pectoral fin distal region
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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