ZFIN ID: ZDB-FIG-200406-194
Pei et al., 2020 - A subset of SMN complex members have a specific role in tissue regeneration via ERBB pathway-mediated proliferation. NPJ Regenerative medicine   5:6 Full text @ NPJ Regen Med
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 6 Reduced responsiveness of <italic>gemin3</italic>, <italic>gemin5</italic>, and <italic>smn1</italic> mutants to ErbB pathway inhibitor AG1478.

a Neuromasts in AG1478-treated control and gemin5hg81 mutant embryos at 5 dpf. Neuromasts are shown as white dots. Scale bar, 250 µm. b Quantification of the lateral line neuromasts in mock and AG1478-treated embryos carrying the gemin5hg81 mutation at 5 dpf. c Quantification of the lateral line neuromasts in mock and AG1478-treated embryos carrying the smn1hg104 mutation at 5 dpf. d Quantification of the lateral line neuromasts in mock and AG1478-treated embryos carrying the gemin3hg105 mutation at 5 dpf. e Quantification of the lateral line neuromasts in mock and AG1478-treated embryos carrying the gemin6hg110 mutation at 5 dpf. Error bars in the graphs show the mean ± s.e.m. ns, P > 0.05; *P < 0.05; **P < 0.01; ****P < 0.0001. f Fluorescent images of lateral line neuromasts labeled by transgenes Tg(pou4f3:GAP-GFP) and Tg(SqET20:EGFP) in the control and gemin5hg81 mutant at 5 dpf after AG1478 treatment. Images were taken in the areas surrounding the end of yolk extension. White arrow points to the Tg(SqET20:EGFP) signal in the control embryo, which is dramatically increased in the mutant. Scale bar, 50 µm. The embryos used for the above analyses were generated from a pairwise incross of heterozygotic parents, treated with 2 µM AG1478 from 1 to 5 dpf, and then used Yopro-1 staining or transgenic fluorescence at 5 dpf to analyze neuromast formation. Data for each condition were generated using ~40 embryos born from a single pair of heterozygous parents.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: tyrphostin AG 1478 Day 5 neuromast increased amount, abnormal
ddx20hg105/hg105 control Day 5 neuromast amount, normal
ddx20hg105/hg105 chemical treatment by environment: tyrphostin AG 1478 Day 5 neuromast amount, ameliorated
gemin5hg81/hg81 control Day 5 neuromast amount, normal
gemin5hg81/hg81 chemical treatment by environment: tyrphostin AG 1478 Day 5 neuromast amount, ameliorated
gemin5hg81/hg81; s273tTg; sqet20Et chemical treatment by environment: tyrphostin AG 1478 Day 5 neuromast hair cell amount, ameliorated
Day 5 neuromast mantle cell amount, ameliorated
gemin6hg110/hg110 control Day 5 neuromast amount, normal
gemin6hg110/hg110 chemical treatment by environment: tyrphostin AG 1478 Day 5 neuromast increased amount, abnormal
s273tTg; sqet20Et chemical treatment by environment: tyrphostin AG 1478 Day 5 neuromast hair cell increased amount, abnormal
Day 5 neuromast mantle cell increased amount, abnormal
smn1hg104/hg104 control Day 5 neuromast amount, normal
smn1hg104/hg104 chemical treatment by environment: tyrphostin AG 1478 Day 5 neuromast amount, ameliorated
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ NPJ Regen Med