FIGURE

Fig. 6

ID

ZDB-FIG-200319-40

Publication

Lanni et al., 2019 - Integrated K+ channel and K+Cl- cotransporter function are required for the coordination of size and proportion during development

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Fig. 6

Allelic titration of slc12a7a/kcc4a expressivity revealing necessity and sensitivity to levels of Kcc4a during development. A) Identified deletions in F1 progeny and predicted protein alteration; blue box, sequence targeted by guide RNA. B) Representative pictures of individuals heterozygous for different slc12a7a/kcc4a alleles. C) Barbel phenotypes conferred by different slc12a7a/kcc4a alleles. D) Schematic of Kcc4a protein depicting locations of premature stop codon, 3 amino acid deletion, 6 amino acid deletion (red asterisk), and schleier mutation (yellow asterisk). E) Tabulation of relative length of fins and barbels in F1 progeny heterozygous for each genotype; mean ± SEM is shown. n.s., not significant; *p < 0.05; **p < 0.01 calculated using a one-way ANOVA followed by a Tukey’s multiple comparisons test; n = 6–19 fish per genotype.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc12a7a^zf3270/+ slc12a7a^zf3271/+
Observed In:	barbel fin
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 456(2), Lanni, J.S., Peal, D., Ekstrom, L., Chen, H., Stanclift, C., Bowen, M.E., Mercado, A., Gamba, G., Kahle, K.T., Harris, M.P., Integrated K+ channel and K+Cl- cotransporter function are required for the coordination of size and proportion during development, 164-178, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.