FIGURE 1

Gene expression domains demarcate alar and basal territories within the secondary prosencephalon as well as tuberal and mamillary subregions within the basal hypothalamus. (A–F″) Sagittal optical sections of zebrafish embryos 48 hpf at medial, ventricular or more lateral, mantle positions as indicated, stained by double-fluorescent whole-mount in situ hybridization using probes as indicated. (G) Integration of several prevalent anatomical models of the zebrafish embryonic forebrain referencing orientation colinear to the body axis (Wullimann et al., 1996; Manoli and Driever, 2014; Biran et al., 2015; Mueller and Wullimann, 2016; Muthu et al., 2016). (H) Proposed model of hypothalamus subdivision into mamillary and tuberal areas at ventricular (H′) or mantle zones (H″) with anatomic directions colinear to the neuraxis. Dashed box in (A″) indicates area which is shown in higher magnification in Supplementary Figure S2A. Arrowhead in (A″) indicates the rostral border of the floor plate. The alar-basal boundary is shown in cyan. Maximum intensity projections of 10 (A–A″), 15 (C–C″), 25 (B–B″,D–D″,F–F″), or 35 (E–E″) 1 μm confocal planes. Abbreviations see list. Scale bar 50 μm.