FIGURE

Fig 2

ID

ZDB-FIG-200229-21

Publication

Concilio et al., 2020 - Inter-species variation in monovalent anion substrate selectivity and inhibitor sensitivity in the sodium iodide symporter (NIS)

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Fig 2

Characterization of HA-NIS protein expression and iodide transport.

Species are ordered in ascending evolutionary proximity to humans as determined by TimeTree (pig, mouse, rat, and dog diverged equidistantly) [73]. (A) Percentage of 1.25 μg/ml puromycin-selected cells which were positively-stained with α-HA-AlexaFluor647 antibody. (B) Median fluorescence intensity of 1.25 μg/ml puromycin-selected cells which were stained with α-HA-AlexaFluor647 antibody. Values are averages of duplicate assays. (C) Absolute uptake value of each NIS protein in the absence or presence of 100 μM perchlorate (ClO₄), which has not been equalized to protein expression. Values are averages of triplicate assays with standard deviation. (D) Percentage of maximum ¹²⁵I^- uptake maintained in the presence of 100 μM perchlorate. Untrans. = untransduced, Ab = antibody.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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