FIGURE

Figure 3

ID

ZDB-FIG-200212-48

Publication

Idigo et al., 2020 - Translation elongation factor 1A2 (eEF1A2) is encoded by one of four eef1a genes and is dispensable for survival in zebrafish

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Figure 3

Expression analysis of the zebrafish <italic>eef1a</italic> genes

(A) Expression of eef1a1l1 in different early embryonic stages detected by RT-PCR. The other eef1a genes were undetected at these stages (data not shown). (B) Expression of eef1a genes in 24, 48 and 72 hpf developmental stages using RT-PCR. NTC, no template control. (C) Expression of eef1a genes in different tissues of adult zebrafish detected by RT-PCR. (D) Expression levels of eef1a genes in brain, muscle and liver tissues, and (E) comparison of the relative levels of their transcripts in these tissues. Expression values were normalised to those of ATPsynth, NADH and 16S. Results are means ± SD, n=3. *P<0.05; **P<0.01; ***P<0.0001. For comparison, data were presented as the gene expression ratio of the target mRNA to the geometric mean of reference genes for each tissue. (F) Western blot showing Eef1a2 expression in brain, liver and muscle zebrafish tissues using eEF1A2-Abcam antibody (1:1000). Control is muscle tissue from mouse. (G) Validation of eEF1A2-Abcam antibody (ab82912) specificity using lysates isolated from individually transfected HEK293T cells with GFP-tagged Eef1a constructs. Abbreviation: Zf, zebrafish.

Expression Data

Genes:	eef1a1a eef1a1b eef1a1l1 eef1a2
Antibody:	Ab2-eef1a2
Fish:	AB
Anatomical Terms:	brain intestine liver muscle ovary spleen testis whole organism
Stage Range:	1-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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