FIGURE 3

Immunostaining of zpr-1, zpr-2, and zpr-3 in slc7a14-deficient morphants. (A) In the peripheral retina, the high-dose slc7a14-MOs led to sharp reductions in zpr-3. Weaker fluorescence signals were detected in the high-MO dose groups (slc7a14 4.0 ng and slc7a14 6.0 ng) than in the control group. (B) Statistical results for zpr-3 (n = 10 for each group). (C) The high-dose slc7a14-MOs led to significant reductions in zpr-2. There were few fluorescence signals in the peripheral retina in the high-MO dose group (slc7a14 6.0 ng). The RPE in the low-MO dose group (slc7a14 2.0 ng) was relatively normal compared to that in the wild-type group and control group. (D) Statistical results for zpr-2 (n = 10 for each group). (E) No significant changes were found in cone photoreceptors. (F) Statistical results for zpr-1 (n = 5 for each group). Scale bar = 50 μm. Bar plots were shown as the mean ± s.e.m. T-test was performed between the two groups. ^∗P < 0.05, ^∗∗P < 0.01.