FIGURE

Figure 6

ID

ZDB-FIG-191230-798

Publication

Castro-Sánchez et al., 2019 - Functional analysis of new human Bardet-Biedl syndrome loci specific variants in the zebrafish model

Other Figures

All Figure Page

Back to All Figure Page

Figure 6

Representative images of KVs and comparison of average cilia length corresponding to BBS5 conditions. Projections of confocal z-stacks of Alexa fluor 594 and Dapi stained cilia located in Kupffer’s vesicle (KV) from control (A) and bbs5 MO-injected embryos (B), bbs5 MO-injected embryos plus WT capped-mRNA (C) and bbs5 MO-injected embryos plus BBS5 new changes (R138C, F180fs, N184S) capped-mRNA. (D–F) Note the reduction of the cilia in bbs5 MO and BBS5 variants R138C and N184S capped-mRNA co-injected embryos. (G) Comparison of average cilia length. *p < 0.01, **p < 0.001. Scales bars: 10 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-bbs5
Knockdown Reagent:	MO1-bbs5
Observed In:	Kupffer's vesicle cilium
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.