ZFIN ID: ZDB-FIG-191230-666
Pérez-Rius et al., 2019 - Comparison of zebrafish and mice knockouts for Megalencephalic Leukoencephalopathy proteins indicates that GlialCAM/MLC1 forms a functional unit. Orphanet journal of rare diseases   14:268 Full text @ Orphanet J Rare Dis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 1

Generation of a glialcama knockout line in zebrafish. a An allele with a deletion of 7 nucleotides in glialcama (Δ7, from now on −/−) was generated using a TALEN nuclease. The deletion generates an early stop codon, resulting in a protein of only 28 amino acids in lenght. b Western blot of brain extracts from adult wild-type (+/+), heterozygous (+/−) or homozygous mutant (−/−) fish for the glialcama knockout allele demonstrates the absence of the glialcama protein in the homozygous zebrafish. c glialcama expression in the optic tract (Ot) (arrowheads) and diffusely in cell bodies of the preoptic region (asterisk) of wild type fish (dotted line: optic tract/preoptic region boundary). d No glialcama immunoreactivity is observed in the optic tract or brain parenchyma of glialcama knockout fish. Dotted line: optic tract/preoptic region boundary. Asterisk: preoptic region. e glialcama immunoreactivity is observed in the inner limiting membrane of the wild type retina (arrowheads). f No glialcama immunoreactivity is observed in knockout retina. Expression observed in glialcama−/− represents autofluorescence in photoreceptors that was consistently observed, even in the case of control immunostainings with secondary antibody only. Arrows point to the inner limiting membrane. Scale bars: 60 μm (e, f); 75 μm (c, d)

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hepacama Ab1-hepacama WT standard conditions Adult brain WB
Ab1-hepacama Adult inner limiting membrane IHC
Ab1-hepacama Adult Muller cell glial limiting end-foot IHC
Ab1-hepacama Adult optic tract IHC
Ab1-hepacama Adult preoptic area IHC
Ab1-hepacama Adult radial glial cell glial cell projection IHC
Ab1-hepacama hepacamabcn1/bcn1 standard conditions Adult Not Detected brain WB
Ab1-hepacama Adult Not Detected inner limiting membrane IHC
Ab1-hepacama Adult Not Detected Muller cell glial limiting end-foot IHC
Ab1-hepacama Adult Not Detected optic tract IHC
Ab1-hepacama Adult Not Detected preoptic area IHC
Ab1-hepacama Adult Not Detected radial glial cell glial cell projection IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hepacamabcn1/bcn1 standard conditions Adult brain hepacama expression absent, abnormal
Adult inner limiting membrane hepacama expression absent, abnormal
Adult Muller cell glial limiting end-foot hepacama expression absent, abnormal
Adult optic tract hepacama expression absent, abnormal
Adult preoptic area hepacama expression absent, abnormal
Adult radial glial cell glial cell projection hepacama expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Orphanet journal of rare diseases for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Orphanet J Rare Dis