FIGURE

Fig. 5.

ID

ZDB-FIG-191230-485

Publication

Gurevich et al., 2019 - Live imaging the Foreign Body Response reveals how dampening inflammation reduces fibrosis

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Fig. 5.

Genetic immunosuppression results in decreased TNFα expression and reduced avascular zone. (A) Representative images of csf1ra^−/− Tg(tnfα:GFP); Tg(mpeg:mCherry) double transgenic adult zebrafish immediately prior to and following suture pull through (PT, top row, white circle), or implantation of nylon (middle row, red circle) or vicryl suture (bottom row, red braided circle), at indicated time points. The area of wounding or implantation is marked by a white asterisk; the dotted lines indicate nylon suture. n=6 independent fish per condition. (B) Quantification of the mean±s.d. altered inflammatory area surrounding the wound/suture, measured from images in A. (C) Representative images of Tg(fli:GFP) transgenic adult zebrafish immediately prior to and following suture pull through (top row, white circle), or implantation of nylon suture (middle row, red circle) or vicryl suture (bottom row, red braided circle), at indicated time points. The area of wounding or implantation is marked by a white asterisk; the dotted lines indicate nylon suture. n=6 independent fish per condition. (D) Quantification of mean±s.d. avascular zone immediately adjacent to wound/suture, measured from images in C. Scale bars: 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	csf1ra^j4e1/j4e1; gl23Tg; pd1028Tg csf1ra^j4e1/j4e1; y1Tg (TL)
Condition:	surgical manipulation: trunk
Observed In:	angiogenesis involved in wound healing inflammatory response to wounding
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.