ZFIN ID: ZDB-FIG-191230-485
Gurevich et al., 2019 - Live imaging the Foreign Body Response reveals how dampening inflammation reduces fibrosis. Journal of Cell Science   133(5) Full text @ J. Cell Sci.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 5.

Genetic immunosuppression results in decreased TNFα expression and reduced avascular zone. (A) Representative images of csf1ra−/− Tg(tnfα:GFP); Tg(mpeg:mCherry) double transgenic adult zebrafish immediately prior to and following suture pull through (PT, top row, white circle), or implantation of nylon (middle row, red circle) or vicryl suture (bottom row, red braided circle), at indicated time points. The area of wounding or implantation is marked by a white asterisk; the dotted lines indicate nylon suture. n=6 independent fish per condition. (B) Quantification of the mean±s.d. altered inflammatory area surrounding the wound/suture, measured from images in A. (C) Representative images of Tg(fli:GFP) transgenic adult zebrafish immediately prior to and following suture pull through (top row, white circle), or implantation of nylon suture (middle row, red circle) or vicryl suture (bottom row, red braided circle), at indicated time points. The area of wounding or implantation is marked by a white asterisk; the dotted lines indicate nylon suture. n=6 independent fish per condition. (D) Quantification of mean±s.d. avascular zone immediately adjacent to wound/suture, measured from images in C. Scale bars: 200 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
csf1raj4e1/j4e1; gl23Tg; pd1028Tg surgical manipulation: trunk Adult inflammatory response to wounding decreased occurrence, abnormal
csf1raj4e1/j4e1; y1Tg(TL) surgical manipulation: trunk Adult angiogenesis involved in wound healing occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.