FIGURE

Figure 3

ID

ZDB-FIG-191230-295

Publication

Novoa et al., 2019 - Rag1 immunodeficiency-induced early aging and senescence in zebrafish are dependent on chronic inflammation and oxidative stress

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

Rag1^−/− zebrafish present with histological defects and alterations compared with wt siblings. (a–c) Premature aging signals in rag1^−/− zebrafish. Representative images of retina (a) and liver (b, c) sections from 1‐year‐old wt and rag1^−/− zebrafish (n = 5). (a) H&E‐stained eye sections revealed the loss of the photoreceptor layer and reduced thickness of nuclear layers. (b) The liver presents with hepatocyte cytoplasmic vacuolization. (c) Liver sections reveal numerous PAS‐positive areas, suggesting cytosolic accumulation of aging biomarker lipofuscin in hepatocytes (arrows). (d–i) Macrophage infiltration in rag1^−/− zebrafish. Representative images of retina (d, g), skin (e, h), and muscle (f, i) sections from 1‐year‐old rag1^−/− zebrafish. Consecutive anti‐Lcp‐ and anti‐Mpx‐immunostained sections reveal strong macrophage infiltration (Lcp⁺/Mpx^‐) in the different tissues examined

Expression Data

Antibodies:	Ab1-il1b Ab5-mpx
Fish:	WT rag1^{t26683/t26683}
Anatomical Terms:	integument leukocyte integument neutrophil muscle leukocyte muscle neutrophil retina leukocyte retina neutrophil
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rag1^{t26683/t26683}
Observed In:	integument leukocyte integument macrophage integument neutrophil muscle leukocyte muscle macrophage muscle neutrophil retina leukocyte retina macrophage retina neutrophil
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Aging Cell