ZFIN ID: ZDB-FIG-191230-1827
O'Brown et al., 2019 - Suppression of transcytosis regulates zebrafish blood-brain barrier function. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Adult

Figure 4 (A) FISH for mfsd2aa (left), mfsd2ab (middle) and mouse Gfap negative control (right) in Tg(kdrl:mCherry) 3 dpf, 5 dpf and adult brain tissue. FISH for mfsd2aa (left) reveals no vascular expression above background at 3 dpf, high levels at 5 dpf and low levels in adult blood vessels. FISH for mfsd2ab (middle) reveals high levels of vascular signal at 3 and 5 dpf and negligible vascular signal in adults. Neither Mfsd2a paralogue was expressed exclusively in the vasculature, outlined in white, at any time examined and mfsd2ab displayed higher overall expression throughout the larval brain than mfsd2aa. Scale bar represents 10 ┬Ám. (B) Quantification of the number of transcript puncta per blood vessel area in 3 dpf, 5 dpf and adult brain sections after background (mouse Gfap expression) subtraction. N = 6 sections from at least 3 different fish.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry s896Tg standard conditions Protruding-mouth brain vasculature ISH
Day 5 brain vasculature ISH
Adult brain vasculature ISH
mfsd2aa s896Tg standard conditions Protruding-mouth brain ISH
Day 5 brain ISH
Day 5 brain vasculature ISH
Adult brain ISH
Adult brain vasculature ISH
mfsd2ab s896Tg standard conditions Protruding-mouth brain ISH
Protruding-mouth brain vasculature ISH
Day 5 brain ISH
Day 5 brain vasculature ISH
Adult brain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife