FIGURE

Figure 5

ID

ZDB-FIG-191230-1366

Publication

Palasin et al., 2019 - Abnormal development of zebrafish after knockout and knockdown of ribosomal protein L10a

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Figure 5

(a) Significant reductions in hemoglobin (Hb) staining in the embryos were observed after rpl10a gene knockdown (MO^aug and MO^sp), similar to rps19 knockdown, compared to the control; embryos co-injected with rpl10a transcript recovered at 48 hpf. (b) rpl10a embryos mutated using CRISPR-Cas9 knockout showed significant reductions in hemoglobin staining. (+/+: wild-type, +/−: heterozygous, −/−: homozygous mutant). The graph displayed the percentages of normal and abnormal levels of Hb staining in (c) knocked down rpl10a gene and mRNA rescue embryos; (d) 5 bp rpl10a mutant embryos. The dense Hb staining (orange dot) at the wild-type embryo yolk sac was considered normal while a significantly pale orange or slightly colored Hb staining in the yolk sac was evaluated as abnormal. The number of animals quantified in each group are shown on top of the bars.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rpl10a^myz1/myz1 (AB) AB + MO1-rpl10a AB + MO2-rpl10a rpl10a^myz1/+ (AB)
Knockdown Reagents:	MO1-rpl10a MO2-rpl10a
Observed In:	whole organism hemoglobin
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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