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ZFIN ID: ZDB-PUB-191204-19
Abnormal development of zebrafish after knockout and knockdown of ribosomal protein L10a
Palasin, K., Uechi, T., Yoshihama, M., Srisowanna, N., Choijookhuu, N., Hishikawa, Y., Kenmochi, N., Chotigeat, W.
Date: 2019
Source: Scientific Reports   9: 18130 (Journal)
Registered Authors: Kenmochi, Naoya, Uechi, Tamayo
Keywords: none
MeSH Terms:
  • Animals
  • CRISPR-Cas Systems
  • DEAD-box RNA Helicases/genetics
  • Embryo, Nonmammalian/abnormalities*
  • Erythropoiesis/genetics
  • GATA1 Transcription Factor/genetics
  • Gene Expression Regulation, Developmental*
  • Gene Knockdown Techniques
  • Gene Knockout Techniques
  • Germ Cells/physiology
  • Hemoglobins/genetics*
  • Oligonucleotides, Antisense
  • RNA-Binding Proteins/genetics*
  • RNA-Binding Proteins/metabolism
  • Tumor Suppressor Protein p53/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 31792295 Full text @ Sci. Rep.
In this study, to investigate the secondary function of Rpl10a in zebrafish development, morpholino antisense oligonucleotides (MOs) were used to knock down the zebrafish ribosomal protein L10a (rpl10a). At 25 hpf (hours post-fertilization), embryos injected with the rpl10a MO showed an abnormal morphology, including short bodies, curved tails, and small yolk sac extensions. We observed pigment reductions, edema, larger yolk sacs, smaller eyes and smaller yolk sac extensions at 50 hpf. In addition, reductions in the expression of primordial germ cell (PGC) marker genes (nanos1 and vasa) were observed in rpl10a knockdown embryos. A rescue experiment using a rpl10a mRNA co-injection showed the recovery of the morphology and red blood cell production similar to wild-type. Moreover, the CRISPR-Cas9 system was used to edit the sequence of rpl10a exon 5, resulting in a homozygous 5-bp deletion in the zebrafish genome. The mutant embryos displayed a morphology similar to that of the knockdown animals. Furthermore, the loss of rpl10a function led to reduced expression of gata1, hbae3, and hbbe1 (erythroid synthesis) and increased tp53 expression. Overall, the results suggested that Rpl10a deficiency caused delays in embryonic development, as well as apoptosis and anemia, in zebrafish.