FIGURE

Figure S3

ID

ZDB-FIG-191230-1307

Publication

Roca et al., 2019 - TNF Induces Pathogenic Programmed Macrophage Necrosis in Tuberculosis through a Mitochondrial-Lysosomal-Endoplasmic Reticulum Circuit

Other Figures

All Figure Page

Back to All Figure Page

Figure S3

TNF Triggers Mitochondrial ROS Production Only in Infected Macrophages, Related to Figure 5

(A) Representative confocal images of 1 dpi TNF-high or control larvae with yellow fluorescent macrophages, showing MitoTracker Red CM-H₂Xros fluorescence corresponding to similar area of the fish as in Figure 3A. Detail: macrophage in the orange rectangle. Scale bar 10 μm.

(B) Quantification of mitochondrial ROS production in 1 dpi TNF-high or control larvae. Each point represents the mean of maximum intensity fluorescence of MitoTracker Red CM-H₂Xros per fish from images in (A). Black and red symbols represent uninfected and Mm-infected macrophages, respectively, in the same control or TNF-administered animal. Horizontal bars, means; ^∗∗∗p < 0.001 (one-way ANOVA with Tukey’s post-test).

(C) Representative confocal images of 1 dpi TNF-high or control larvae showing MitoSOX fluorescence (red) corresponding to similar area of the fish as in Figure 3A. Detail: macrophage in the orange rectangle. Scale bar 10 μm.

(D) Quantification of mitochondrial ROS production in 1 dpi TNF-high or control larvae. Each point represents the mean of maximum intensity fluorescence of MitoSOX per fish from images in (C). Black and red symbols represent uninfected and Mm-infected macrophages, respectively, in the same control or TNF-administered animal. Horizontal bars, means; ^∗∗∗p < 0.001 (one-way ANOVA with Tukey’s post-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 178(6), Roca, F.J., Whitworth, L.J., Redmond, S., Jones, A.A., Ramakrishnan, L., TNF Induces Pathogenic Programmed Macrophage Necrosis in Tuberculosis through a Mitochondrial-Lysosomal-Endoplasmic Reticulum Circuit, 1344-1361.e11, Copyright (2019) with permission from Elsevier. Full text @ Cell