FIGURE

Figure 6

ID

ZDB-FIG-191230-1227

Publication

Jin et al., 2019 - Zebrafish TRIM25 Promotes Innate Immune Response to RGNNV Infection by Targeting 2CARD and RD Regions of RIG-I for K63-Linked Ubiquitination

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Figure 6

zbTRIM25-mediated K63 ubiquitination in 2CARD and RD regions of zbRIG-I is important for IFN induction. (A) HEK 293T cells were transfected with pEGFP-zbRIG-I, pEGFP-zbRIG-I-2CARD, and pEGFP-zbRIG-I-RD as well as DrIFN1 pro-Luc and pRL-TK vectors. (B,C) HEK 293T cells were transfected with pEGFP-zbRIG-I-2CARD or pEGFP-zbRIG-I-RD together with HA-K63Ub and increasing amount of pCMV-Flag-zbTRIM25 as well as DrIFN1 pro-Luc and pRL-TK plasmids. Luciferase activities were measured and normalized to the amount of Renilla Luciferase activities. Data represent the mean + SD (n = 3). Asterisks indicate significant differences between groups (*p < 0.05; **p < 0.01).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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