Fig. 8
- ID
- ZDB-FIG-191105-2
- Publication
- Schulz et al., 2019 - Analysis of the genomic architecture of a complex trait locus in hypertensive rat models links Tmem63c to kidney damage
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Ultrastructural and morphological analysis of glomerular structures after loss of tmem63c in zebrafish.( A?E) Electron microscopy and quantitative assessment of GFB ultrastructure. Representative electron microscopy pictures of the GFB in uninjected Controls ( A), Cas9-Controls ( B) and after tmem63c knockdown ( C), asterisks indicate effaced podocyte foot processes). Quantitative analysis of podocyte foot process width ( D) and number of slit diaphragms per µm GBM ( E). Uninjected Control ( n = 5); Cas9-Control ( n = 4); tmem63c ex2-sgRNA ( n = 4); Scale bar = 1 µm; values plottet: mean ±SD; One-way ANOVA with Bonferroni?s multiple comparisons test; *p=0.0019 vs. uninjected Control, *p=0.0017 vs Cas9-Control; # p=0.0171 vs. uninjected Control , # p=0.0148 vs. Cas9-Control. ( F?J) Confocal microscopy and analysis of absolute and relative podocyte cell number in Tg(wt1b:EGFP) at 96 hpf. Representative confocal microscopy pictures of glomeruli in uninjected Controls ( F), Cas9-Controls ( G) and after tmem63c knockdown ( H). Quantitative analysis of absolute ( I) and relative ( J) podocyte cell number. Relative podocyte cell number has been obtained after normalization to the glomerular volume. Uninjected Control ( n = 6); Cas9-Control ( n = 6); tmem63c ex2-sgRNA ( n = 7); Scale bar = 15 µm; values plottet: mean ±SD; One-way ANOVA with Bonferroni?s multiple comparisons test; **p=0.0421 vs. uninjected Control. |
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Stage: | Day 4 |
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Stage Range: | Day 4 to Day 5 |